平码二中二怎么算买中
Location: Home > Products > Our Products > RNAi 實驗 > microRNA 原位雜交偵測?microRNA 北方墨點偵測 > miRCURY LNA Detection Control Probes

友善列印miRCURY LNA Detection Control Probes

  • 針對 microRNA 的 ISH (in situ hybridization) 與 Northern Blot 偵測實驗,所設計的實驗對照組探針,同樣採用鎖核酸 (Locked Nucleic Acid, LNA™) 技術,可作為 miRCURY LNA™ microRNA Detection Probe 的實驗對照組。

  • 具有 Positive 與 Negative control probe 可選:

    • U6 small nuclear RNA control probe: Positive control,針對 Human、Mouse、Rat 來源樣品所設計,亦可適用於其他物種來源樣品,唯需先以 BLAST 序列比對軟體確認其可用性。

    • Scramble-miR control probe: Negative control,與 miRBase 中所有 microRNA 序列皆不互補;且與 NCBI 資料庫中的所有核酸序列相同性 (sequence homology) 不超過 70%。

    • Sense miR-159 control probe: Negative control,與 miRBase 中所有 microRNA 序列皆不互補;且與 NCBI 資料庫中的所有非植物核酸序列相同性 (sequence homology) 不超過 70%。不建議使用於植物來源樣品。

     

產品效能:

Detection of hsa-miR-127 and hsa-miR-154 by fluorescent in situ hybridization using miRCURY LNA™ detection probes in cryopreserved bone marrow cells from an Acute Myeloid Leukemia patient. Panels A and B show the cytoplasmic detection of miR-127 and miR-154, respectively. Panel C shows the nuclear expression of the positive control U6 small RNA as visualized using the miRCURY LNA™ U6 control probe. No signal was detected when cells were hybridized with the miRCURY LNA™ scramble probe as a negative control, as seen in panel D. Data used with kind permission from Dr. Silvana Debernardi, Institute of Cancer, London, UK. Original figure appears in Dixon-McIver A, East P, Mein CA, Cazier J-B, Molloy G, et al. (2008) Distinctive Patterns of MicroRNA Expression Associated with Karyotype in Acute Myeloid Leukaemia. PLoS ONE 3(5): e2141. doi:10.1371/journal.pone.0002141.

 

訂購資訊

Product Description Cat. No.
U6
U6, hsa/mmu/rno Control, miRCURY LNA™ detection probe, 1 nmol, ready to label EX-699002-300
U6. hsa/mmu/rno Control, miRCURY LNA™ detection probe, 1 nmol, 5'-DIG labeled EX-699002-340
U6, hsa/mmu/rno Control, miRCURY LNA™ detection probe, 1 nmol, 5'-DIG and 3'-DIG labeled EX-699002-360
U6, hsa/mmu/rno Control, miRCURY LNA™ detection probe, 1 nmol, 5'-fluorescein labeled EX-699002-310
U6, hsa/mmu/rno Control, miRCURY LNA™ detection probe, 1 nmol, 5'-fluorescein and 3'-fluorescein labeled EX-699002-330
U6, hsa/mmu/rno Control, miRCURY LNA™ detection probe, 1 nmol, 5'-biotin labeled EX-699002-370
U6, hsa/mmu/rno Control, miRCURY LNA™ detection probe, 1 nmol, 3'-DIG labeled EX-699002-350
Scramble-miR
Scramble-miR Control, miRCURY LNA™ detection probe, 1 nmol, ready to label EX-699003-300
Scramble-miR Control, miRCURY LNA™ detection probe, 1 nmol, 5'-DIG labeled EX-699003-340
Scramble-miR Control, miRCURY LNA™ detection probe, 1 nmol, 5'-DIG and 3'-DIG labeled EX-699003-360
Scramble-miR Control, miRCURY LNA™ detection probe, 1 nmol, 5'-fluorescein labeled EX-699003-310
Scramble-miR Control, miRCURY LNA™ detection probe, 1 nmol, 5'-fluorescein and 3'-fluorescein labeled EX-699003-330
Scramble-miR Control, miRCURY LNA™ detection probe, 1 nmol, 5'-biotin labeled EX-699003-370
Scramble-miR Control, miRCURY LNA™ detection probe, 1 nmol, 3'-DIG labeled EX-699003-350
Sense miR-159
Sense miR-159 Control, miRCURY LNA™ detection probe, 1 nmol, ready to label EX-699004-300
Sense miR-159 Control, miRCURY LNA™ detection probe, 1 nmol, 5'-DIG labeled EX-699004-340
Sense miR-159 Control, miRCURY LNA™ detection probe, 1 nmol, 5'-DIG and 3'-DIG labeled EX-699004-360
Sense miR-159 Control, miRCURY LNA™ detection probe, 1 nmol, 5'-fluorescein labeled EX-699004-310
Sense miR-159 Control, miRCURY LNA™ detection probe, 1 nmol, 5'-fluorescein and 3'-fluorescein labeled EX-699004-330
Sense miR-159 Control, miRCURY LNA™ detection probe, 1 nmol, 5'-biotin labeled EX-699004-370
Sense miR-159 Control, miRCURY LNA™ detection probe, 1 nmol, 3'-DIG labeled EX-699004-350

 

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平码二中二怎么算买中
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